Preparation 0.004 M Benzethonium Chloride
Weigh 1.792gm of benzethonium chloride (dried to constant weight at 105˚C) in a 1000 ml volumetric flask add sufficient water to dissolve properly and dilute to 1000 ml with water. Standardize the solution in the following manner.
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Standardarzition 0.004 M Benzethonium Chloride
Dissolve 0.35 gm of the dried substance (benzethonium chloride) in 30 ml of anhydrous glacial acetic acid, add 6ml of mercuric acetate solution and carry out the non-aqueous titration (appendix 3.45, IP), using 0.05 ml of crystal violet solution as indicator. Perform a blank determination and any necessary corrections.
Each ml of 0.1M perchloric acid is equivalent to 0.04481gm of C27H42ClNO2
Calculation 0.004 M Benzethonium Chloride
Molarity: = (W) x (P)/(W1) x 100
Where W = Actual Weight of benzethonium chloride used for preparing Volumetric Solution
W1= Molecular Weight of benzethonium chloride
P = Potency of benzethonium chloride
The potency of benzethonium Chloride (P): = Vol. of HClO4 x Molarity of HClO4 ×100 × 44.81 / Weight of benzethonium Chloride × 0.1
ACCEPTANCE CRITERIA
1) The accuracy of the solution should be within ± 10% of the theoretical concentration.
2) RSD* of the triplicate results of standardization / re-standardization should not be more than 0.2 %.
| Reference | I P/In-House (IP 2007, Vol. I, Page No. 548) |
|---|---|
| Frequency Preparation | 1 Month |
| Standardization | Initially after preparation |
| Re-standardization | After 15 days |
| Storage conditions | Store the solution in a well-stoppered glass bottle at ambient temperature |
| Precautions (if any) | None specific |